Analyzing the Transcriptome Profile of Human Cumulus Cells Related to Embryo Quality via RNA Sequencing

Table 1

Primers used for qRT-PCR.

cDNA	Sense Primer (5’-3’)	Antisense Primer (5’-3’)
MSTN	ACTGGTGTGGCAAGTTGTCT	TGGACCAGCAACAATCAGCA
CTGF	GAGAGTCCTTCCAGAGCAGC	CATAGTTGGGTCTGGGCCAA
NDUFA1	GCGTACATCCACAGGTTCAC	ACTCCAGAGATGCGCCTATC
SCD5	ATCTTCGAGTGGTCCAGGGA	TGACGTCAAGCTTTCTCCCC
VCAN	TGACTGTGGATGGGGTTGTG	GCGTCACACTGCTCAAATCC
STAR	TCCTTGACCCCTTCCTTTGC	GCAGCATTGTTTCCTGGCAA
GAPDH	CCAGGGCTGCTTTTAACTCTG	TGACAAGCTTCCCGTTCTCA

MSTN, myostatin; CTGF, connective tissue growth factor; NDUFA1, ubiquinone oxidoreductase subunit A1; VCAN, versican; SCD5, stearoyl-CoA desaturase 5; STAR, steroidogenic acute regulatory protein; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.