Ghrelin Ameliorates Angiotensin II-Induced Myocardial Fibrosis by Upregulating Peroxisome Proliferator-Activated Receptor Gamma in Young Male Rats

Figure 5

Ghrelin reduced cell viability of adult rat cardiac fibroblasts stimulated with Ang II. Cell viability was measured by MTT assay. (a) Cardiac fibroblasts were pretreated with Ghrelin (1, 10, 100, or 1000 nM) for 24 h, followed by stimulation with Ang II (100 nM) for 24 h. (b) Cardiac fibroblasts were pretreated with Ghrelin (100 nM) for 3, 6, 12, or 24 h, followed by stimulation with Ang II (100 nM) for 24 h. (c) CFs were pretreated with or without Ghrelin or Ghrelin plus GW9662 followed by stimulated with or without Ang II for 24h. Results are expressed as mean ± SEM, n = 6 each per group, p<0.05 versus Control; ^#p<0.05 versus Ang II; ^&p<0.05 versus Ang II+Ghrelin.

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