GAS1 Deficient Enhances UPR Activity in Saccharomyces cerevisiae

Figure 1

GAS1 deficiency decreases the growth rates and RLS of yeast cells. (a) Relative transcription level of GAS1 mRNA in the GAS1 deficient (gas1Δ) and GAS1 overexpression (GAS1 OE) strains. The data are expressed as the means ± SD (n=3). p < 0.01 vs. BY4742. (b) Spore progenies were grown into colonies from single cells on YPD plates after backcrossing. (c) Yeast cells were serially diluted (1:10) and cultured on YPD plates. (d) Growths of the BY4742, gas1Δ, and GAS1 OE strains were monitored (OD₆₀₀) at various time points. (e) RLS curves of the BY4742, gas1Δ, and GAS1 OE strains. Mortality curves were generated from lifespan data, and BY4742 was considered to be the control. Numbers in parentheses are the mean RLS and cell number values.

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