Resveratrol Suppresses Epithelial-Mesenchymal Transition in GBM by Regulating Smad-Dependent Signaling
Figure 2
Resveratrol (Res) suppresses EMT in GBM cells. (a) MTT assay of cell viability in LN18 and U87 cells following resveratrol treatment for 48 hours. (b) Representative morphological images were photographed under Olympus microscope for EMT-induced LN18 and U87 cells treated with or without resveratrol for 48 hours (× 100 magnification). (c) Western blotting showed that resveratrol inhibited EMT-induced downregulation of E-cadherin and suppressed EMT-induced upregulation of N-cadherin, β-catenin, Vimentin, Snail, Slug, and Twist1 expression in a dose-dependent manner. All samples were analyzed in triplicate. (d) Immunofluorescence showed that resveratrol inhibited EMT-induced downregulation of E-cadherin and upregulation of Vimentin (× 400 magnification).