Resveratrol Suppresses Epithelial-Mesenchymal Transition in GBM by Regulating Smad-Dependent Signaling
Figure 3
Resveratrol (Res) inhibits EMT-induced migration and invasion of GBM cells. (a) Resveratrol (40μM) inhibited EMT-induced migratory ability in LN18 and U87 GBM cells. Representative wound-healing images were photographed under Olympus microscope for LN18 (× 100 magnification) and U87 (× 40 magnification) GBM cells. The mean levels of migration distance observed in three random fields for each condition were showed in histograms. (b) Resveratrol (40μM) inhibited EMT-induced invasive ability in LN18 and U87 GBM cells. Representative transwell images were photographed under Olympus microscope for LN18 (× 100 magnification) and U87 (× 100 magnification) GBM cells. The mean levels of the numbers of cells counted in five random fields on each filter for each condition were showed in histograms. Each independent experiment was repeated three times. (c) Western blotting showed that resveratrol inhibited EMT-induced upregulation of MMP-2 and MMP-9 expression in a dose-dependent manner.