Research Article
Combined Activation of Guanylate Cyclase and Cyclic AMP in Lung Fibroblasts as a Novel Therapeutic Concept for Lung Fibrosis
Figure 7
Blockade of β-catenin expression in fibroblasts by sGC and cAMP activation. (a) Transcription of β-catenin was inhibited by LY2157299, BAY 41-2272, and forskolin (all at 10−6 M) in TGF-β1 stimulated fibroblasts. Bars represent mean ±SEM of triplicate experiments. (b) Representative immunochemical staining for β-catenin in unstimulated cells (left upper panel), TGF-β1 (5 ng/ml) stimulated fibroblasts (right upper panel), and cells preincubated (30 min) with increasing concentrations of either BAY 41-2272 or forskolin followed by TGF-β1-stimulation (5 ng/ml). Similar results were obtained in three additional experiments.
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