The Dosage of the Derivative of Clostridium Ghonii (DCG) Spores Dictates Whether an IFNγ/IL-9 or a Strong IFNγ Response Is Elicited in TC-1 Tumour Bearing Mice
Figure 1
Intravenous administration of DCG does not increase the numbers of T cells in the spleen of TC-1 tumour bearing mice. Group of 5-7 six-week-old female C57/BL6 mice were inoculated with 2x106 of TC-1 tumour cells subcutaneously. When tumour grew to 300 mm3 in size, tumour bearing mice were injected intravenously with either (A) 1x108 CFU/kg amount of CG spores, (B) 3x108 CFU/kg amount of CG spores, or (C) PBS or received (D) Nil treatment; no treatment was given to the tumour bearing mice. 11 days after CG spore injection, mice were sacrificed and spleens were isolated and single cell suspension was made by physical disruption of spleen described in Materials and Methods. Single spleen cells were stained with relevant antibodies and run through flow cytometer. Live cells were gated first; then CD45+ cells were gated. The results were analysed by NovoExpress™. (a) Numbers of CD45+ cells, (b) F4/80+; (c) NK1.1+, (d) B220+; (e) CD3+; (f) CD4+, (g) CD8+ T cells. P<0.05 is considered statistically significant. The result represents one of two independent experiments.