The Dosage of the Derivative of Clostridium Ghonii (DCG) Spores Dictates Whether an IFNγ/IL-9 or a Strong IFNγ Response Is Elicited in TC-1 Tumour Bearing Mice
Figure 3
Intravenous administration of CG does not significantly increase the numbers of T cells infiltrating to the tumour site. Group of 5-6 six-week-old female C57/BL6 mice were inoculated with 2x106 of TC-1 tumour cells subcutaneously. When tumour grew to 300 mm3 in size, tumour bearing mice were injected intravenously with either (A) 1x108 CFU/kg amount of DCG spores, (B) 3x108 CFU/kg amount of DCG spores, or (C) PBS or received (D) Nil treatment. 11 days after DCG spore injection, mice were sacrificed and tumours were isolated and single cells were made by digest with Collagenase D and cells were stained with anti-CD45. Some cells were subject to Ficoll separation to enrich mononuclear cells in tumour cells, and they were then stained with anti-CD45, anti-CD3, anti-CD4, anti-CD8a, anti-NK1.1, anti-B220, and anti-F4/80. CD45+ T cells were gated. (a) The percentages of CD45+ T cells of live cells; (b) CD3+; (c) CD3+CD4+; (d) CD3+CD8+ T cells; (e) B220+; (f) NK1.1+; (g) F4/80+ cells. P<0.05 is considered statistically significant. Results shown represent one of two independent experiments.