Methylation Changes of Primary Tumors, Monolayer, and Spheroid Tissue Culture Environments in Malignant Melanoma and Breast Carcinoma

Figure 3

Methylation rates of malignant melanoma patients: methylation rates of PAX5 gene were observed between 22 and 68% and 37 and 51% for TMPRSS2 gene, respectively. Methylation in PAX5 gene was seen in all malignant melanoma primary tissue and also grew tumor cells in 2D and 3D tissue culture systems (mg08-mg015). Two of 6 patients with malignant melanoma in 2D and 3D tissue culture systems did not show methylation for TMPRSS2 gene (mg08 and mg013). 2D: two-dimensional tissue culture, 3D: three-dimensional tissue culture, TD: primary tumor tissue, and mg: malignant melanoma.