Research Article
Methylation Changes of Primary Tumors, Monolayer, and Spheroid Tissue Culture Environments in Malignant Melanoma and Breast Carcinoma
Table 1
Methylated primers and probes.
| GENES | SBDS (138bp) | TMPRSS2 (189bp) | PAX5 (230bp) |
| FORWARD PRIMERS | 5′-TGTAGTAGGCGATT | 5′- AG GGA TTT TGG GGC | 5′ AAA GCG TTC GAA | TCGAAGCGT 3′ | GTT TTT CG -3′ | GGT ATC GTG A -3′ |
| PROBES | 6-FAM-GGGGGTGAAGAT | 6-FAM-TCGAGGGTTCGA | 6-FAM-AGC GCG TCG | CGATATCGCGGTT- | GTTTGGGTTAAGGA - | TTT TAT ATT GTC GGT | TAMRA | TAMRA | TCG G - TAMRA |
| REVERSE PRIMERS | 5′- TCA ACC CTA ATT | 5′- AAA ACG AAA CCG | 5′TAC TAC TCT CCC GAC | CGC CGA CTT CT -3′ | ACC GCC TA -3′ | TTC CCG-3′ |
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