Methylation Changes of Primary Tumors, Monolayer, and Spheroid Tissue Culture Environments in Malignant Melanoma and Breast Carcinoma

Table 1

Methylated primers and probes.

GENES	SBDS (138bp)	TMPRSS2 (189bp)	PAX5 (230bp)
FORWARD PRIMERS	5′-TGTAGTAGGCGATT	5′- AG GGA TTT TGG GGC	5′ AAA GCG TTC GAA
	TCGAAGCGT 3′	GTT TTT CG -3′	GGT ATC GTG A -3′
PROBES	6-FAM-GGGGGTGAAGAT	6-FAM-TCGAGGGTTCGA	6-FAM-AGC GCG TCG
	CGATATCGCGGTT-	GTTTGGGTTAAGGA -	TTT TAT ATT GTC GGT
	TAMRA	TAMRA	TCG G - TAMRA
REVERSE PRIMERS	5′- TCA ACC CTA ATT	5′- AAA ACG AAA CCG	5′TAC TAC TCT CCC GAC
	CGC CGA CTT CT -3′	ACC GCC TA -3′	TTC CCG-3′