Research Article
Methylation Changes of Primary Tumors, Monolayer, and Spheroid Tissue Culture Environments in Malignant Melanoma and Breast Carcinoma
Table 2
Unmethylated primers and probes.
| GENES | SBDS (138bp) | TMPRSS2 (189bp) | PAX5 (230bp) |
| FORWARD PRIMERS | 5′ TTT GTA GTA GGT | 5′- ATA AGG GAT TTT | 5′ AGT GTT TGA AGG | GAT TTT GAA GTG 3′ | GGG GTG TTT TTT G -3′ | TAT TGT GAA ATG -3′ |
| PROBES | 6-FAM-TGG TGG GGG | 6-FAM-TGT TTG AGG | 6-FAM-AGT GTG TTG | TGA AGA TTG ATA TTG | GTT TGA GTT TGG GTT | TTT TAT ATT GTT GGT | TGG – TAMRA | AA - TAMRA | TTG GA - TAMRA |
| REVERSE PRIMERS | 5′- AAT CAA CCC TAA | 5′- AAA ACC AAA ACA | 5′- TCT CCC AAC TTC | TTC ACC AAC TT -3′ | AAA CCA ACC ACC -3′ | CCA CTC TA -3′ |
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