Methylation Changes of Primary Tumors, Monolayer, and Spheroid Tissue Culture Environments in Malignant Melanoma and Breast Carcinoma

Table 2

Unmethylated primers and probes.

GENES	SBDS (138bp)	TMPRSS2 (189bp)	PAX5 (230bp)
FORWARD PRIMERS	5′ TTT GTA GTA GGT	5′- ATA AGG GAT TTT	5′ AGT GTT TGA AGG
	GAT TTT GAA GTG 3′	GGG GTG TTT TTT G -3′	TAT TGT GAA ATG -3′
PROBES	6-FAM-TGG TGG GGG	6-FAM-TGT TTG AGG	6-FAM-AGT GTG TTG
	TGA AGA TTG ATA TTG	GTT TGA GTT TGG GTT	TTT TAT ATT GTT GGT
	TGG – TAMRA	AA - TAMRA	TTG GA - TAMRA
REVERSE PRIMERS	5′- AAT CAA CCC TAA	5′- AAA ACC AAA ACA	5′- TCT CCC AAC TTC
	TTC ACC AAC TT -3′	AAA CCA ACC ACC -3′	CCA CTC TA -3′