Methylation Changes of Primary Tumors, Monolayer, and Spheroid Tissue Culture Environments in Malignant Melanoma and Breast Carcinoma

Table 5

Methylation status of genes according to groups of patients and cultivation methods (2D: two-dimensional tissue culture, 3D: three-dimensional tissue culture, and T: primary tumor tissue).

Genes	Patients	Cell culture method	Total number of patients	Absence of Methylation		Presence of Methylation
				Number of patients (n)		Number of patients (n)
PAX5	Breast ca	2D	10	10	100	0	0
		3D	10	9	90	1	10
		Primary tumor tissue	10	3	30	7	70
	Melanoma	2D	6	0	0	6	100
		3D	6	0	0	6	100
		Primary tumor tissue	6	0	0	6	100
TMPRSS2	Breast ca	2D	10	8	80	2	20
		3D	10	8	80	2	20
		Primary tumor tissue	10	4	40	6	60
	Melanoma	2D	6	2	33	4	67
		3D	6	2	33	4	67
		Primary tumor tissue	6	1	17	5	83
SBDS	Breast ca	2D	10	10	100	0	0
		3D	10	10	100	0	0
		Primary tumor	10	10	100	0	0
		tissue
	Melanoma	2D	6	6	100	0	0
		3D	6	6	100	0	0
		Primary tumor tissue	6	5	83	1	17