Dihydroartemisinin Protects against Dextran Sulfate Sodium-Induced Colitis in Mice through Inhibiting the PI3K/AKT and NF-κB Signaling Pathways

Figure 5

Dihydroartemisinin blocks activation of the NF-kB signaling pathway in colitis both in vivo and in vitro. The mice were treated with DSS for 7 days, and IEC-6 cells were treated with LPS for 24 hours, and then, the colon tissue and IEC-6 cell lysates were analyzed by western blotting. (a) Representative image of p-IKK, IKK, p-IkBa, IkBa, and NF-kB (p65) in colon tissues. (b) Quantification analysis of p-IKK/IKK, p-IkBa/IkBa, and NF-kB in colon tissues. N = 4 in each group. vs. control group. vs. DSS group. (c) Representative image of p-IKK, IKK, p-IkBa, IkBa, and NF-kB (p65) in IEC-6 cells. (d) Quantification analysis of p-IKK/IKK, p-IkBa/IkBa, and NF-kB in IEC-6 cells. N = 4 in each group. (e) Relative mRNA expression of TNF-α, IL-6, and IL-1β in IEC-6 cells. N = 4 in each group. vs. control group; vs. LPS group; & vs. LPS + DHA group.

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