TFIIIB Subunit Bdp1 Participates in RNA Polymerase III Transcription in the Protozoan Parasite Leishmania major

Figure 4

Growth curves and Western blot analysis of LmBdp1 knockout parasites. (a) Growth of promastigotes from wild-type (WT), single-knockout (SKO), and double-knockout +1 (DKO+1) cell lines. Values represent means of three experiments. Standard deviation bars are shown. (b) Western blot analysis of LmBdp1 in wild-type (WT) and double-knockout +1 (DKO+1) parasites using the LmBdp1 antibody. The bands shown here and from two more independent assays were quantified and plotted (lower graph). Protein levels of LmBdp1 were normalized to the amount of α-tubulin, which was used as loading control. Standard deviation bars are shown.

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