Bax Targeted by miR-29a Regulates Chondrocyte Apoptosis in Osteoarthritis

Figure 3

Bax is the direct target of miR-29a. (a) Predicted miR-29a seed matches the sequence of Bax 3’UTR. The complementary sequences are shown in green and blue as indicated. (b) Schematic representation of the genomic organization of the human miR-29. (c) Chondrocyte cell cultures of cartilage tissues from control or OA patients were subjected to quantitative PCR (qPCR) and the relative expression levels of miR-29a, miR-29b, and miR-29c were shown. donates p < 0.05 and ns donates no significant differences, both compared to NC group. (d) Wild type (Bax-WT) and seed mutated (Bax-MUT) 3’UTR s of Bax and mature wild type (miR-29a-WT) and seed mutated (miR-29a MUT) were shown. (e) Cultured ATDC5 cell was cotransfected with dual luciferase reporter with wild type miR-29a together with Bax-WT or Bax-MUT. The relative activity was represented. donates p < 0.05, compared with vector control. (f) Dual luciferase activity assays by cotransfection with wild type Bax 3’UTR together with miR-29a-WT or miR-29a-MUT expression plasmids. donates p < 0.05, compared with vector control.

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