Research Article

Improving Ethyl Acetate Production in Baijiu Manufacture by Wickerhamomyces anomalus and Saccharomyces cerevisiae Mixed Culture Fermentations

Table 3

The volatile compounds in the different simulated solid-state fermentations (μg/kg).

Volatile compoundsABCDOdor thresholds (g/L)

Ethanol521±4723±7535±11150±3100,000
Isoamyl alcohol27±1133±2--179,190.83
β-Phenethyl alcohol695±133,436±2453±119±28,922.73
1-Octen-3-ol12±1115±2236±9732±246.12
2-Phenyl-propan-2-ol-166±935±1-/
  Higher alcohols1,2554,573859901
 Ethyl acetate833±9625±764±1-32,551.60
 Ethyl caprylate57±176±345±2-12.87
 Ethyl caprate-23±22±-1122.30
 Ethyl phenylacetate39±2182±8--406.83
 Ethyl isovalerate-73±27--6.89
 Dimethyl phthalate18±23±1-/
 Ethyl tetradecanoate34±87±2--800
 Dibutyl phthalate64±322±10--/
 Ethyl pentadecanoate-87±23--7,000
 Ethyl 9-hexadecenoic acid88±3--1,500
 Ethyl palmitate584±101,632±20--2,000
 Ethyl oleate587±101,563±21--/
  Esters2,2164,776154-
 2-Octanone583±10861±23464±6288±9/
 Acetophenone157±836±2--/
 3-Octanone-62±133±143±1/
  Ketones740959497431
 Methyl catechol981±32,361±19-57±323
 4-Vinylguaiacol2,153±1294±487±2-1,100
 4-Ethylguaiacol-11,609±2923±164±233
  Phenols3,13414,064110121
 Isooctanoic acid13±21±111±-/
 Isovaleric acid-93±17--1045.47
  Acids1311411-
 Azulene109±2253±523±127±6/
 Styrene23±114±2--80
 1-Caryophyllene114±3108±6--/
  Alkenes24647523127
 Pentadecane32±44±2-74±3/
 Hexadecane23±52±118±1-300,000-400,000
 Eicosane18±1--/
  Alkanes641141874
 Phenylacetaldehyde-22±13±-5.2
Sum7,66825,0751,6721,654

A: Only S. cerevisiae Y3401 and W. anomalus Y3604 were in the solid-state fermentations system; B: S. cerevisiae Y3401, W. anomalus Y3604 and Daqu were in the solid-state fermentations system; C, Only Daqu was in the solid-state fermentations system; D, no inoculation was in the solid-state fermentations system.
Note: Data are average of three replicates ± standard deviations; “-”, not detected; Same lowercase letters in each line do not differ significantly at 5% probability by Duncan’s multiple range tests; “/”, not available.