Rs2015 Polymorphism in miRNA Target Site of Sirtuin2 Gene Is Associated with the Risk of Parkinson’s Disease in Chinese Han Population
Figure 2
The screening of the target miRNA on the 3′UTR within rs2015 of SIRT2 gene and the influence of rs2015 on the binding efficiency in vitro. (a) Diagram of the prediction of the miRNA-binding site of SIRT2 3′UTR within SNP rs2015. (b, c, d) Luciferase reporter constructs containing SIRT2 3′UTR or its mutants were cotransfected with three corresponding miRNA mimics (miR-4760-5P, miR-8061, and miR-376a) in HEK-293T cells. (e, f) The expression level of corresponding miRNAs after transfected mimics or inhibitors of miRNAs to the SH-SY5Y cell by QRT-PCR assay, respectively. (g, h) The mRNA expression level of SIRT2 with the corresponding miRNAs’ intervention in SH-SY5Y cell by QRT-PCR assay, respectively. (i, j) Western blot analysis for SIRT2 expression treated with mimics or inhibitors of the corresponding miRNA in SH-SY5Y cells. Data represent the mean ± SEM of three or four independent experiments. P<0.05 vs. control group, P<0.01 vs. control group, P<0.0001 vs. control group.