Long Noncoding RNA MALAT1 Acts as a Competing Endogenous RNA to Regulate TGF-β2 Induced Epithelial-Mesenchymal Transition of Lens Epithelial Cells by a MicroRNA-26a-Dependent Mechanism

Figure 2

TGF-β2 induced the expression of EMT markers in primary HLECs via a MALAT1-dependent mechanism. (a, b, c) The primary HLECs were treated with TGF-β2 (5 ng/ml) for 48 h before incubation with MALAT1 siRNAs or control siRNA for 24 h. (a) E-cadherin and fibronectin protein levels in primary HLECs transfected and treated as indicated were detected by Western blot analysis. (b) E-cadherin mRNA levels in primary HLECs were detected by qRT-PCR. (c) Fibronectin mRNA expression in primary HLECs was detected using qRT-PCR. P < 0.05 compared with normal ((b) and (c)). ^#P < 0.05 compared with group with TGF-β2 ((b) and (c)). All of data are presented as the mean± SE of six independent experiments.

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