Long Noncoding RNA MALAT1 Acts as a Competing Endogenous RNA to Regulate TGF-β2 Induced Epithelial-Mesenchymal Transition of Lens Epithelial Cells by a MicroRNA-26a-Dependent Mechanism
Figure 6
LncRNA MALAT1/Smad4 axis is involved in TGF-β2 induced EMT of LECs. (a, b, c) The primary HLECs were treated with TGF-β2 (5 ng/ml) for 48 h before incubation with MALAT1 siRNAs or Smad4 siRNA for 24 h. (a) E-cadherin and fibronectin protein levels in primary HLECs were detected by Western blot analysis. (b) E-cadherin mRNA levels in primary HLECs were detected by qRT-PCR. (c) Fibronectin mRNA expression in primary HLECs was detected using qRT-PCR. P < 0.05 compared with TGF-β2 group ((b) and (c)). #P < 0.05 compared with TGF-β2 group and TGF-β2+MALAT1 siRNAs group ((b) and (c)). ##P < 0.05 compared with TGF-β2+MALAT1 siRNAs group and TGF-β2+MALAT1 siRNAs+Smad4 siRNAs group ((b) and (c)). (d, e, f) The primary HLECs were treated with TGF-β2 (5 ng/ml) for 48 h before incubation with pcDNA3.1-MALAT1 vector or Smad4 siRNA for 24 h. (d) E-cadherin and fibronectin protein levels in primary HLECs were detected by Western blot analysis. (e) E-cadherin mRNA levels in primary HLECs were detected by qRT-PCR. (f) Fibronectin mRNA expression in primary HLECs was detected using qRT-PCR. P < 0.05 compared with normal group and pcDNA3.1-control group ((e) and (f)). #P < 0.05 compared with pcDNA3.1-MALAT1 group ((e) and (f)). All of data are presented as the mean± SE of six independent experiments.