Preventive CTLA-4-Ig Treatment Reduces Hepatic Egg Load and Hepatic Fibrosis in Schistosoma mansoni-Infected Mice

Figure 4

Cytokine production by splenocytes and expression of cytokines in the livers of mice preventively treated with CTLA-4-Ig are impaired. (a) Splenocytes were isolated and stimulated with 10 μg/ml SWAP (soluble worm antigen preparation). Supernatants were collected after 72 h and amounts of IL-13, IL-4, INF-γ, and IL-10 (n = 4–9 out of 3 independent experiments) were quantified by ELISA (n = 4–9). (b) Relative gene expression of Il13, Il4, Ifng, and Il10 in livers of mice was determined by real-time PCR (n = 6–9). Data are represented as mean + SD from duplicate data.

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