A Noninvasive Prediction Nomogram for Lymph Node Metastasis of Hepatocellular Carcinoma Based on Serum Long Noncoding RNAs

Table 1

Primer sequences designed for qRT-PCR.

Gene ID	Forward primer	Reverse primer	Product length	Tm (°C)
lnc-GALR2-1:1	5′-CTGAGCACCAAAAGTCTGGC-3′	5′-GCACTCCCATTGTTCGGGAT-3′	90	60
ENST00000418803	5′-GCATAGGCATAAATTAGGATCTGGAGA-3′	5′-TGGAAGGGAGGGGAGTGGAT-3′	128	60
lnc-ZNF35-4:1	5′-GAGTGAGTTTGTGGACCCAGATTTC-3′	5′-GGTCATGTTATATGCTTGCCTTCAG-3′	111	60
lnc-EPS15L1-2:1	5′-TCCCCAACTTTCTGAGACTGT-3′	5′-AAGGAAGTGTGATCCGAGAGGT-3′	133	60
lnc-CAMKK2-3:2	5′-GGATGCCCTAAAGGACTCCG-3′	5′-CCTCCAGATGGCCGTTTTCT-3′	94	60