Rapid and Simple Detection of Trichosporon asahii by Optimized Colony PCR

Figure 1

The sensitivity of colony PCR detection based on different methods. M: DNA Ladder; 1-7: Suspension of the type strain of T. asahii CBS2479, Concentration followed by 10⁷, 10⁶, 10⁵, 10⁴, 10³, 10², 10¹ CFU/mL; 8: Negative control; 1(a): glass beads; 1(b): freeze-thawing; 1(c): direct colony; 1(d): enzymolysis; 1(e): traditional DNA extraction.

(a)

(b)

(c)

(d)

(e)