BioMed Research International

Research Article

Rapid and Simple Detection of Trichosporon asahii by Optimized Colony PCR

Table 1

Strains used in this study and the specificity evaluation of species-specific primers for detecting T. asahii.
OrganismStrainSpecificity detection
TA1TA2TA3TA4
Trichosporon asahiiBZP7001++++
Trichosporon asahiiBZP7002++++
Trichosporon asahiiBZP7003++++
Trichosporon asahiiBZP7004++++
Trichosporon asahiiBZP7005++++
Trichosporon asahiiBZP9001++++
Trichosporon asahiiBZP9002++++
Trichosporon asahiiCBS2479++++
Trichosporon asahiiBZP4848++++
Trichosporon asahiiBZP6108++++
Trichosporon asahiiBZP6198++++
Trichosporon asahiiBZP6674++++
Trichosporon asahiiCBS7137++++
Trichosporon asahiiCBS8520++++
Trichosporon asahiiCBS8904++++
Trichosporon debeurumanianum CBS1896−−−−
Trichosporon dermatis CBS2043−−−−
Trichosporon cutaneum CBS2466−−−−
Trichosporon asteroides CBS2481−++−
Trichosporon coremiiforme CBS2482−++−
Trichosporon faecale CBS4828−++−
Trichosporon inkin CBS5585−−−−
Trichosporon jirovecii CBS6864−−−−
Trichosporon ovoides CBS7556−−−−
Trichosporon mucoides CBS7625−−−−
Trichosporon montevideense CBS8261−++−
Trichosporon domesticum CBS8280−−−−
Trichosporon japonicum CBS8641−+−−
Trichosporon lactis CBS9051−−−−
Trichosporon dohaense CBS10761−−−−
Candida dubliniensisCMCC(F)C8f−−−−
Candida tropicalisCMCC(F)C2f−−−−
Candida parapsilosisCMCC(F)C4f−−−−
Candida glabrataCMCC(F)T10a−−−−
Candida metapsilosisCMCC(F)C4k−−−−
Candida albicansATCC90028−−−−
Cryptococcus neoformansCMCC(F)D2a−−−−
Cryptococcus gattiiCMCC(F)D2e−−+−
Trichophyton rubrumCMCC(F)T1h−−−−
 Trichophyton violaceumBZP1501−−−−
Trichophyton mentagrophytesCMCC(F)T5e−−−−
Malassezia furfurCMCC(F)T17a−−−−
Malassezia sympodialisCMCC(F)T14a−−−−
Sporothrix schenkiiCMCC(F)D1a−−−−
Microsporum gypseumCMCC(F)M2b−−−−
Microsporum canisCMCC(F)M3h+−−−
Aspergillus versicolorCMCC(F)A5c−−−−
Aspergillus fumigatusCMCC(F)A1g+−−−
Aspergillus nigerCMCC(F)A3a−−−−
Aspergillus nidulansCMCC(F)A7c−−−−
Fonsecaea pedrosoiCMCC(F)D6j+−−−
Phialophora verrucosaCMCC(F)D8i−−−−
Scedosporium apiospermumCMCC(F)D13f−−−−
Penicillium marneffeiCMCC(F)B33a−−−−
Rhizopus oryzaeCMCC(F)B81c−−−−
Mucor circinelloidesCMCC(F)B57a−−−−
Fusarium oxysporumCMCC(F)B38c−−−−
 Absidia CorymbiferaCMCC(F)B69f−−−−
Staphylococcus aureusClinical isolates−−−−
Staphylococcus epidermidisClinical isolates−−−−
Streptococcus pneumoniaeClinical isolates−−−−
Streptococcus pyogenesClinical isolates−−−−
Klebsiella pneumoniaClinical isolates−−−−
Escherichia coliClinical isolates−−−−
Acinetobacter baumanniiClinical isolates−−−−
Enterococcus faeciumClinical isolates−−−−
 Serratia odoriferaClinical isolates−−−−
Healthy human DNA −−−−
Negative control −−−−
ATCC: American type culture collection, Rockville, MD, USA; CBS: Centraalbureau voor Schimmelcultures, Baarn, The Netherlands; CMCC: China Medical Culture Collection, Nanjing, China; BZP: The Military Institute of Injury and Reparation, China.