Rapid and Simple Detection of Trichosporon asahii by Optimized Colony PCR

Table 2

Sensitivities, positive rate of trace detection, and procedure duration of different colony PCR methods for detecting T. asahii.

Methods	Sensitivity	Positive rate of trace detection	Procedure duration
			Pretreatment	PCR	Electrophoresis	Total
glass beads	10^2CFU/mL	100% (15/15)	4.8±0.6min			154.8±0.6min
freeze-thawing	10^2CFU/mL	73.33% (11/15)	32.7±1.5min			182.7±1.5min
direct colony	10^3CFU/mL	73.33% (11/15)	1±0.3min	100min	50min	151±0.3min
enzymolysis	10^3CFU/mL	93.33% (14/15)	60.6±0.4min			210.6±0.4min
traditional DNA extraction	10^3CFU/mL	53.33% (8/15)	295.4±7.9min			445.4±7.9min