AVE 0991 Attenuates Pyroptosis and Liver Damage after Heatstroke by Inhibiting the ROS-NLRP3 Inflammatory Signalling Pathway
The expression of renin-angiotensin system after the onset of heatstroke in rats. (a–i) Samples of serum and liver sections were obtained from the sham rats and heatstroke rats at the time-point of heatstroke onset (0 h) and 4 and 9 h after heatstroke onset. The serum levels of Ang II (a), Ang-(1-7) (b), ACE (c), and ACE2 (d) were analysed by ELISA. The liver contents of Ang II (e) and Ang-(1-7) (f) were analysed by ELISA. (g) Protein expression of the AT1 receptor and the MAS receptor in liver tissue was analysed by western blotting. The quantification of the relative protein expression is shown in the graph (right). (h) Real-time PCR analysis of the AT1 receptor and the MAS receptor mRNA expression in liver tissue. (i) Immunohistochemical staining was performed to analyse AT1 and MAS protein expression. P<0.05 compared with the control group. The data are presented as the mean ± SD. All of the assays were performed in triplicate. ACE: angiotensin converting enzyme; ACE2: angiotensin-converting enzyme 2; AT1: angiotensin II type 1 receptor.
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