Prediction and Structural Comparison of Deleterious Coding Nonsynonymous Single Nucleotide Polymorphisms (nsSNPs) in Human LEP Gene Associated with Obesity

Table 3

Prediction of change in protein stability using I-MUTANT 2 and Mupro.

Uploaded variation	AA position	Imutant-2		MUPRO
		DDG		DDG		SVM		NN
rs866158426	S46L	−0.49	Decrease	−0.417	Decrease	−0.476	Decrease	−0.982	Decrease
rs200575914	G59S	−1.15	Decrease	−0.911	Decrease	−0.782	Decrease	−0.693	Decrease
rs886061972	D61N	−1.06	Decrease	−0.813	Decrease	−0.259	Decrease	−0.761	Decrease
rs724159998	D100N	−2.68	Decrease	−1.09	Decrease	−0.781	Decrease	−0.725	Decrease
rs28954113	N103K	−0.7	Decrease	−1.282	Decrease	−0.73	Decrease	−0.663	Decrease
rs1051206328	C117S	−0.98	Decrease	−1.852	Decrease	−1	Decrease	−0.912	Decrease
rs771956117	L161R	−0.29	Decrease	−2.214	Decrease	−1	Decrease	−0.964	Decrease
rs104894023	R105W	−1.02	Decrease	−1.22	Decrease	−1	Decrease	0.90	Decrease