Clinical Features and Cytokine Profile in Myositis Patients with Anti-EJ Autoantibodies Detected by a Novel Immunoprecipitation Assay

<div>Establishment of unlabeled protein immunoprecipitation assay using HEK293 lysate overexpressing GlyRS with flag tag for detecting EJ autoantibody. (a) Overexpression of GlyRS in HEK293 cells by transient transfection of pENTER-flag- GlyRS plasmid was confirmed by immunoblot using flag antibody. (b) IP using HEK293 lysate overexpressing GlyRS with flag tag and sera from reference positive serum or healthy control serum.</div>

BioMed Research International

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Figure 1

Figure 1: Clinical Features and Cytokine Profile in Myositis Patients with Anti-EJ Autoantibodies Detected by a Novel Immunoprecipitation Assay 