Microenvironment of Mycobacterium smegmatis Culture to Induce Cholesterol Consumption Does Cell Wall Remodeling and Enables the Formation of Granuloma-Like Structures
Mycobacterium smegmatis modifies its growth rate in minimal medium. Growth rate () in Middlebrook 7H9 broth and (a) minimal medium (MM) with or without glycerol and/or cholesterol supplementation or (b) only in MM with or without B12 vitamin (B12) and/or asparagine (Asn). Viable cells after culture in MM with or without B12 and/or Asn were identified after reculturing each group in complete Middlebrook 7H9 broth (c).
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