Muscle Atrophy Marker Expression Differs between Rotary Cell Culture System and Animal Studies

Table 4

Fold change in gene expression of atrophy-indicating mRNAs from this in vitro study (ULA T25 12 days + RCCS 3 days) compared to referenced work in vivo, as measured by qPCR. ns = not significant, p<0.01, p<0.001.

	In Vitro	In Vivo	In Vivo method
MuRF1	1.3	1.9, 1.6 [22]	Casting 3 days, tenotomy 3 days
MAFbx	1.3	2.7, 1.5 [22]	Casting 3 days, tenotomy 3 days
Caspase 3	3.8	1.0, 3.3 [22]	Casting 3 days, tenotomy 3 days
Ankrd1	1.1	3.9 [27]	Hind limb unloading 6 days
Foxo3	1.4	1.6 [27]	Hind limb unloading 6 days