MicroRNA Profiling of Exosomes Derived from Red Blood Cell Units: Implications in Transfusion-Related Immunomodulation

Table 2

List of primers used in qRT-PCR.

Gene	Primers	Annealing temperature(°C)	Product length (bp)
U6	F:5’GCTTCGGCAGCACATATACTAAAAT3’; R:5’CGCTTCACGAATTTGCGTGTCAT3’	60	89
miR-4454	GSP:5’GGCACGATCCGAGTCACG3’; R:5’GTGCGTGTCGTGGAGTCG3’	60	62
miR-451a	GSP:5'GGGGGAAACCGTTACCATTAC3’; R:5’GTGCGTGTCGTGGAGTCG3’	60	65
miR-125b-5p	GSP:5’GCTCCCTGAGACCCTAAC3’; R:5’GTGCGTGTCGTGGAGTCG3’	60	62