The Influence of Oxidative Stress and Natural Antioxidants on Morphometric Parameters of Red Blood Cells, the Hemoglobin Oxygen Binding Capacity, and the Activity of Antioxidant Enzymes

Figure 8

Ratio of Raman spectrum characteristic bands of Hb in erythrocytes when exposed to AOs during oxidative stress. is p≤0,05 compared to control samples. Δ is p≤0,05 compared to the OS case. (1) I₁₃₇₅/(I₁₃₅₅+I₁₃₇₅) – the relative amount of HbO₂ in the blood; (2) I₁₃₅₅/I₁₅₅₀ – the ability of the total Hb in the sample to bind ligands; (3) I₁₃₇₅/I1₅₈₀ – the ability of Hb to isolate ligands; (4) (I₁₃₅₅/I₁₅₅₀)/(I₁₃₇₅/I₁₅₈₀) – the affinity of Hb to ligands, primarily to O₂; (5) I₁₃₇₅/I₁₁₇₂ – the severity of symmetric and asymmetric fluctuations and alterations of pyrrole rings.