Biochemical and Biological Profile of Parotoid Secretion of the Amazonian Rhinella marina (Anura: Bufonidae)

Figure 5

(a) Cytotoxic effects of crude venom upon human cells, after 72 h treatment, by MTT assay. Results are expressed as mean percentage in treated cells compared to control (solvent) ± SD of three independent experiments. In detail the IC50 value for each cell line. (b) Induction of DNA strand breaks by crude venom as evaluated by the comet assay in alkaline conditions. Bars represent the mean ± SD of three independent experiments. MMS is used as positive control. ^∗Significant difference as compared to negative control treatment at ^∗∗∗P < 0.001/one-way ANOVA Tukey’s multiple comparison test.

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