Polycystin-1 Inhibits Cell Proliferation through Phosphatase PP2A/B56α

Figure 2

PP2A-dependent regulation of cell proliferation by PC1 in HEK293T cells. (a) Effects of PC1-5TMC on proliferation with treatment of PP2A inhibitors OA or CA (1 nM). Shown are averaged data (N = 4). HEK293T cells transiently expressing GFP-PC1-5TMC or GFP were plated in multiple wells of a 96-well plate after 24 hr of transfection and grown for another 24 hr for cell proliferation assay labelling with or without OA or CA treatment. (b) Effects of WT PC1 on proliferation with treatment of OA or CA (1 nM). HEK293T cells stably expressing flag-tagged full-length PC1 after 24 hr of culture replacing the above cells performed as shown in (a) (N = 4). (c) Effects of full-length PC1 on proliferation with siRNA knockdown of PP2A/B56α. HEK293T cells stably expressing WT PC1 or GFP vector were transiently transfected with B56α siRNA and grown for 24 hr before cell proliferation assay. Left panel, effectiveness of siRNA assessed by WB. Right panel, averaged data (N = 4).

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