The Nutritional Cytokine Leptin Promotes NSCLC by Activating the PI3K/AKT and MAPK/ERK Pathways in NSCLC Cells in a Paracrine Manner

Figure 1

Isolation and characterization of CAFs and NLFs. (a) Three-dimensional model of the novel noncontact coculture device. (b) Morphology of primary fibroblasts under a microscope. Left: CAF, right: NLF. Scale bar = 500 μm. (c) The purity of the primary cells was verified by RT-qPCR to determine the levels of α-SMA, vimentin, and CK19. (d) Leptin expression in primary CAF and NLF cell lines was detected with western blot. The quantification of leptin is shown in the bar chart.

(a)

(b)

(c)

(d)