Extraction and Characterization of Organ Components of the Malaysian Sea Cucumber Holothuria leucospilota Yielded Bioactives Exhibiting Diverse Properties

Table 3

Bioactive components detected and measured via GC-MS indifferent solvent extracts of body organs of sea cucumber, H. leucospilota sampled from Peninsular Malaysia.

RT	Compounds	% of Compounds in extract
		A/CT	MA/CT	A/VO	MAVO	A/BW	MABW	PABW	EDBW	MDBW	PDBW
	Ketones
5.558	4-hydroxy-4-methyl-2-Pentanone,	37	15	18	8.69	27	18.9	2.5	17.7	30.8	34.1
5.533	4-methyl-3-Penten-2-one	0.5	3.82	1.8	1.17	1.9	-	-	-	-	1.22
	Phenols
20.225	2,4-bis(1,1-dimethylethyl)-Phenol-	2.7	1.11	2	1.33	5.9	2.14	-	0.95	1.41	1.73
	Alcohols
13.367	2-Furanmethanol	3.7	1.05	4.7	0.53	1.5	0.42	-	0.18	0.67	0.72
20.783	(+)-trans,trans-5-caranol	3.8	-	-	-	-	-	-	-	-	-
21.017	10-Methyl-8-tetradecen-1-ol-acetate	6.6	-	-	-	-	-	-	-	-	-
22.6	trans-9-Hexadec-en-1-ol	-	9.15	-	-	-	-	-	-	-	0.18
20.35	Glycerin	-	-	0.73	0.5	-	-	0.97	0.49	6.54	-
	Hydrocarbons
5.642	3-Chlorooctane	5.8	3.37	20	4.13	20	4.75	-	1.2	-	3.67
5.808	2-Chlorooctane	6.8	10.5	9.5	2.26	11	4.5	-	3.66	7.28	8.36
8.933	3,4-dimethyl-1-decene	2.5	4.02	3.9	-	1	1.14	-	-	-	-
9.075	3-ethyloctane	-	5.45	-	-	-	-	-	-	-	-
5.592	1- methyl -3-ethyl Cyclopentane	-	11.7	-	-	-	-	-	-	-	-
5.592	Cyclopentane, 1-ethyl-2-methyl	-	-	-	-	5.19	-	-	-	-	-
7.692	1-Nonene	1.1	1.17	-	0.59	0.8	-	-	-	-	-
	Fatty acids
23.117	Tetradecanoic acid	-	-	2	0.21	1.2	-	1.16	1.04	0.93	0.65
25.783	n-Hexadecanoic acid	0.8	0.52	8.8	1.12	6	1.39	7.93	12.7	5.68	3.43
26.217	Z-11-Hexadecanoic acid	-	-	5.7	-	4.1	-	-	-	-	-
26.267	9-Hexadecenoic acid	-	-	3.5	-	2.7	0.79	4.8	3.24	2.41	1.67
29.683	cis-9-Octadecenoic acid (oleic acid)	-	-	0.45	0.77	0.5	-	2.94	1.52	-	-
24.533	Eicosanoic acid (arachidic acid)	-	-	0.17	0.51	-	-	1.32	0.68	0.22	-
27.133	Heptadecanoic acid	-	-	-	-	0.5	-	-	1.72	-	-
RT	Compound	% of Compounds in extract
		A/CT	MA/CT	A/VO	MA/VO	A/BW	MA/BW	PA/BW	PD/BW	ED/BW	MD/BW
	Fatty acids continued.
10.642	Cyclohexane butanoic acid	-	1.06	-	-	-	-	-	-	-	-
28.9	Octadecanoic acid	-	-	1.6	0.2	1.9	0.57	3.54	3.72	1.33	0.85
	Carboxylic acid
10.65	Acetic acid	0.8	-	-	-	0.8	0.36	-	0.82	1.1	0.97
14.717	Crotonic acid (2-butenoic acid)	0.4	0.22	0.52	0.67	0.46	-	-	-	0.16	0.4
	Aldehydes
15.267	2,4- dimethyl benzaldehyde	-	-	1.3	0.83	2.6	0.85	-	0.44	0.91	-
14.225	3,7-dimethyl-2,6-Octadienal	-	-	-	-	-	4.64	-	-	-	-
10.35	Furfural	-	-	-	-	1.8	0.83	-	-	-	-
22.033	(Z)-9- Octadecenal	-	-	-	1.11	1.3	-	-	-	-	-
	Sterols
40.9	Stigmast-5-en-3-ol, (3β, 24σ)- (sitosterol)	-	-	0.5	-	2.54	-	-	-	2.15
47.017	14-Methyl-ergost-8-en-ol, (3β,5α)-	-	-	-	-	-	-	0.77	7.34	-	-
38.558	Stigmasta-5,22-dien-3-ol, acetate, (3β, 22ζ)-	-	-	-	0.85	-	-	-	1.72
31.9	Ergosta-14,22-dien-3-ol, acetate, (3β, 5α, 22ε)-	-	-	-	-	-	-	-	-	1.03	-
30.9	Cholest -3-ene, (5β)	-	1.08	-	-	3.6	3.16	-	-	3.37	3.56
33.858	Cholesta-3,5-diene	-	0.95	-	0.43	0.8	2.63	-	-	2.29	1.96
37.717	Ergosta-4, 22-diene	-	-	-	-	-	1.98	-	-	-	-
35.592	Ergosta-4,6,22-triene	-	-	-	-	0.4	-	-	-	-	1.21
	Fatty acid methyl/ethyl esters
19.592	9-Hexadecenoic acid methyl ester	-	3.36	0.32	12.8	1.3	3.61	-	-	-	-
19.308	Hexadecanoic acid methyl ester	-	7.86	-	17.63	-	9.05	0.24	-	-	-
21.225	Octadecanoic acid methyl ester	0.6	7.41	0.44	8.35	1.1	4.82	-	-	0.25	0.33
21.483	13- Octadecenoic acid, methyl ester	-	1.96	-	3.8	-	1.72	-	-	-	-
23.15	7-Hexadecenoic acid methyl ester	-	3.91	-	2.12	-	2.12	-	0.13	-	0.17
17.783	Pentadecanoic acid methyl ester	-	0.33	-	4.07	-	1.72	-	-	-	-
19.575	Hexadecenoic acid methyl ester	-	-	-	-	-	6.35	-	-	-	-
RT	Compound	% of Compounds in extract
		A/CT	MA/CT	A/VO	MA/VO	A/BW	MA/BW	PA/BW	PD/BW	ED/BW	MD/BW
	Fatty acid methyl/ethyl esters continued
22.292	Eicosanoic acid methyl ester	-	1.7	-	-	-	1.07	-	-	-	-
34.875	11-Eicosenoic acid methyl ester	-	0.44	0.2	0.75	-	0.14	21.7	12.1	6.07	3.59
17.233	Methyl tetradecanoate	0.9	0.87	-	3.37	-	1.21	-	-	-	-
39.717	5,8,11,14-Eicosatetraenoic acid ethyl ester (arachidonic acid)	-	8.27	-	10.5	1.1	12.7	20.6	11.9	6.04	3.02
40.725	5,8,11,14,17- Eicosapentaenoic acid, methyl ester (EPA)	-	2.55	-	5.57	-	3.68	-	-	-	-
42.842	4,7,10,13,16,17,19-Docosahexaenoic acid, methyl ester (DHA)	-	-	-	-	-	-	2.74	-	-	-
	Esters
31.783	Decanedioic acid, bis (2-ethylhexyl) ester	4	-	-	2.8	-	-	-	-	-	1.14
24.883	Hexanedioic acid, bis (2-ethylhexyl) ester	-	-	3.8	-	-	-	-	-	-	-
17.075	n-tridecyl ester, 2-Propenoic acid	-	9.1	-	-	-	-	-	-	-	-
	Amines
16.017	2-methyl-2-Propanamine	-	-	3.1	0.85	-	-	-	-	-	-
16.308	2,4,4-Trimethyl-2-Pentanamine	-	-	-	-	2.8	-	-	-	-	-

- represents not detectable; A/CT: acetone Cuvierian tubule; MA/CT: methanol/acetone Cuvierian tubule; A/VO: acetone visceral organ; MAVO: methanol/acetone visceral organ; A/BW: acetone body wall; MA/BW: methanol/acetone body tissue; PABW: isopropanol/acetone body tissue; EDBW: ethanol/distilled water body wall; MDBW: methanol/distilled water body wall; PDBW: isopropanol/distilled water body wall extracts.