Effects of Four Compounds from Gentianella acuta (Michx.) Hulten on Hydrogen Peroxide-Induced Injury in H9c2 Cells

Figure 8

Analysis of HO-1, GCLC and Nrf2 protein levels on H9c2 cells. (a) Western blotting analysis HO-1, GCLC, Nrf2 protein expression with or without ZnPP, BSO, and brusatol (HO-1group only added ZnPP, GCLC group only added BSO, Nrf2 group only added brusatol). (b) Western blot analysis HO-1 protein expression with or without ZnPP. (c) Western blot analysis GCLC protein expression with or without BSO. (d) Western blot analysis Nrf2 protein expression with or without brusatol. (e) Effect of the different treatments on H9c2 cells. Data are expressed as the means ± SD, n = 3. < 0.001, < 0.05 compared with control group. ^###P < 0.001, ^##P < 0.01, ^#P < 0.05 compared with H₂O₂ group. < 0.001, < 0.01 compared with H₂O₂ group combined with four compounds. ^&&&P < 0.001, ^&&P < 0.01, ^&P < 0.05 compared with H₂O₂ group combined with inhibitors and four compounds. GAPDH was used as an internal control.

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