Virulence Factor Genes Incidence among Enterococci from Sewage Sludge in Eastern Slovakia following Safety Aspect

Table 1

Oligonucleotides used for amplification of virulence genes in enterococci isolated form sewage sludge.

Primer	Locus	Sequence (5'-3')	bp
(1) Cytolysin	cylA	F:TAGCGAGTTATATCGTTCACTGTA	1282
		R:CTCACCTCTTTGTATTTAAGCATG
(2) ESP^a	esp	F: TTGCTAATGCTAGTCCACGACC	933
		R: GCGGTCAACACTTGCATTGCCGAA
(3) Adhesin EE^b	efaAfs	F:GACAGACCCTCACGAATA	705
		R:AGTTCATCATGCTGTAGTA
(4) Adhesin EF^c	efaAfm	F: AACAGATCCGCATGAATA	735
		R:CATTTCATCATCTGATAGTA
(5) Gelatinase	gelE	F:ACCCCGTATCATTGGTTT	419
		R:ACGCATTGCTTTTCCATC
(6) Hyaluronidase	hylEfm	F: GAGTAGAGGAATATCTTAGC	661
		R: AGGCTCCAATTCTGT
(7) Element IS16	IS16	F:CATGTTCCACGAACCAGAG	547
		R:TCAAAAAGTGGGCTTGGC

(1) Semedo et al. (2003), (2) ^aenterococcal surface protein, Eaton and Gasson (2001), (3) ^badhesin Enterococcus faecalis, Eaton and Gasson (2001), (4) ^cadhesin Enterococcus faecium, Eaton and Gasson (2001), (5) Eaton and Gasson (2001), (6) Klare et al. (2005), (7) Werner et al. (2011).