A New Strategy for the Detection of Chicken Infectious Anemia Virus Contamination in Attenuated Live Vaccine by Droplet Digital PCR

Figure 3

The determination of ddPCR specificity. (a) Peak frequency of positive droplets detected CIAV-SDLY08. (b) Peak frequency of negative droplets detected NDV, IBV, and FPV. (c) The ddPCR results for CIAV, NDV, IBV, and FPV detection. The unbroken pink line is the threshold, above which are positive droplets (blue) with PCR amplification and below which are negative droplets (gray) without any amplification. Four ddPCR reactions with different virus of template are divided by the vertical dotted yellow line. (d) Copy numbers of ddPCR results for CIAV, NDV, IBV, and FPV. (e) The number of positive droplets in CIAV, NDV, IBV, and FPV samples.

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