Research Article

Circular RNA Profiling by Illumina Sequencing via Template-Dependent Multiple Displacement Amplification

Table 1

List of divergent primers designed for use in confirmation of putative circRNA.

Divergent primerSequence

HindIII 10Forward: 5′-CTATAGTTGAAGCACCTGATGGTGT-3′
Reverse: 5′-GAGCCATAAAGATAGGCAGTAACTACA-3′

HindIII 33Forward: 5′-TGGTTCACCACAACCCGT-3′
Reverse: 5′-TGTGTGACTCAAGTTCTCAGTTTGTAA-3′

osi_circ1
(1:36416264-36418547)
Forward: 5′-TGGTAGCAACCGCACAAA-3′
Reverse: 5′-ATGCTTCCAGGCACATCA-3′

osi_circ2
(2:19273316-20009087)
Forward: 5′-GGGAGCTCAAGGTGAAGAT-3′
Reverse: 5′-GTTGAACAAACAACACACAAC-3′

osi_circ3
(8:24552647-24573025)
Forward: 5′-ACGTTGAGAGTAAGTTTCCG-3′
Reverse: 5′-CCCTTTACGATACCACTAGCC-3′

osi_circ4
(12:16650523-17328210)
Forward: 5′-TAGGCTCACGATGTGTTGC-3′
Reverse: 5′-CGATGAGGGCTGCGAAC-3′

osi_circ5
(9:15720676-15721227)
Forward: 5′-ATCCTTGGAGCTGGCTATGA-3′
Reverse: 5′-ATCTCGGTTGACCACACACT-3′

osi_circ6
(7:15534138-15534682)
Forward: 5′-TCAAGTCCGCCGTCAAATC-3′
Reverse: 5′-CCCAAGGGCAGGTTCTTAC-3′

osi_circ7
(6:27117575-27118530)
Forward: 5′-TGCAGAAACAGCATGGTCA-3′
Reverse: 5′-ATAGGGTGCAAACCTGTGAG-3′

osi_circ8
(8:4494958-4495647)
Forward: 5′-AGAGTCTCTGGCAGTCTCC-3′
Reverse: 5′-AACCAGTGACTAGCAACTAAGAA-3′

osi_circ9
(1:41518651-41519075)
Forward: 5′-GCGACCTTACTGCACGAATA-3′
Reverse: 5′-TTGCAAGCGCAACACAAC-3′

osi_circ10
(8:15854661-15861841)
Forward: 5′-GCTAGCAGGGACAGGTTATC-3′
Reverse: 5′-CAGAAGACGTGTGTGCCTAT-3′

Nb_circ1
(Niben101Scf01334:583095–583645)
Forward: 5′-CTGGGTCAGTCCTCCATTT-3′
Reverse: 5′-AGATACGCATGCCTCCAA-3′

Nb_circ2
(Niben101Scf01481:214685-215144)
Forward: 5′-TCAACGTGCTTCCTGAACT-3′
Reverse: 5′-AAATGCTTGGGTCCTACTCC-3′

Nb_circ3
(Niben101Scf01671:738307-738555)
Forward: 5′-TCTTGTCCCAGTCCAGAGA-3′
Reverse: 5′-TGTCTCCGCGTGTTAATGT-3′

Nb_circ4
(Niben101Scf01820:33613-33924)
Forward: 5′-GTTGTGCTCATTCCATTGGG-3′
Reverse: 5′-TGCTTCCTGAGCAAGTTCTG-3′

Nb_circ5
(Niben101Scf01505:317983-318653)
Forward: 5′-CCCAATCCACCTTGATCCTT-3′
Reverse: 5′-CACGACTGGATTTGGCGATA-3′

Nb_circ6
(Niben101Scf32276:10732-11201)
Forward: 5′-TGGGTACCGAAGTGTACTGT-3′
Reverse: 5′-AAACCTTGGACCGAGATCAAAT-3′

Nb_circ7
(Niben101Scf27324:1438–11811)
Forward: 5′-TGAGCCATTCGCAGTTTCA-3′
Reverse: 5′-GGTCGTCTCGTCCCTTCT-3′

Nb_circ8
(Niben101Scf15187:11992–12579)
Forward: 5′-TGGCTAGAATGCGGGTTTC-3′
Reverse: 5′-ATCTTGAAAGTCGTGGTTTCCT-3′

Nb_circ9
(Niben101Scf09703:266605–266722)
Forward: 5′-GCAGTTGGAGACTTTGAGGT-3′
Reverse: 5′-TGCCGCAAGGGTGATATG-3′

Nb_circ10
(Niben101Scf11535:99376–100075)
Forward: 5′-ACAGGTAGTCTGTTCCGACA-3′
Reverse: 5′-AGATGCCGAGGAGTTGGA-3′