Function of Adipose-Derived Mesenchymal Stem Cells in Monocrotaline-Induced Pulmonary Arterial Hypertension through miR-191 via Regulation of BMPR2

<p>Characterization of ASCs and ASCs-Exos. (a) The expression level of CD31, CD45, CD90, and CD105 in ASCs was detected by FACS. (b) Morphology, adipogenic differentiation, and osteogenic differentiation of ASCs cultured in vitro. Scale bars = 100 <i>μ</i>m. (c) Scheme of exosomes isolation by ultracentrifugation. (d) Representative electron microscopy image of ASCs-Exos. Scale bars = 0.2 <i>μ</i>m. (e) Western blot analysis of exosomal markers. ASCs protein was used as control for exosomes characterized. (f) ASCs-Exos were labeled with PKH-67. ASCs-Exos transferring into HPAECs were tracked using a fluorescence microscope. Scale bars = 20 <i>μ</i>m.</p>

BioMed Research International

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Figure 1

Figure 1: Function of Adipose-Derived Mesenchymal Stem Cells in Monocrotaline-Induced Pulmonary Arterial Hypertension through miR-191 via Regulation of BMPR2 

Figure 1 | Function of Adipose-Derived Mesenchymal Stem Cells in Monocrotaline-Induced Pulmonary Arterial Hypertension through miR-191 via Regulation of BMPR2 