Research Article
Up-Regulated MicroRNA-27b Promotes Adipocyte Differentiation via Induction of Acyl-CoA Thioesterase 2 Expression
Figure 4
Knockdown of ACOT2 expression suppresses adipocyte differentiation. (a) Immunoblotting of ACOT2 in control-siRNA and ACOT2-siRNA transfected 3T3-L1 cells after adipocyte differentiation. Representative data from three different images are shown. (b) Oil red O staining of control siRNA and ACOT2 siRNA transfected 3T3-L1 cells after adipocyte differentiation. Representative data from three different images are shown. Scale bars: 100 μm. (c) Intracellularly TG contents of control siRNA and ACOT2 siRNA transfected 3T3-L1 cells were measured. Each column represents the mean ± SEM from 6 independent experiments. ∗Significantly difference than control siRNA by Student’s t-test ().
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