GAS5 Regulates RECK Expression and Inhibits Invasion Potential of HCC Cells by Sponging miR-135b

Figure 5

MMP-2 inhibition contributes to invasion repression caused by GAS5. (a) Huh-7 and HepG2 cells were infected by lentivirus (LV) expressing GAS5. Huh-7 and HepG2 cells were transfected with GAS5 siRNAs. 72h later, the expression of MMP-2 was detected by qRT-PCR. (b) Huh-7 and HepG2 cells were infected by lentivirus (LV) expressing GAS5. Huh-7 and HepG2 cells were transfected with GAS5 siRNAs. 72h later, the expressions of RECK and MMP-2 was detected by western blot. (c) Huh-7 and HepG2 cells were infected by lentivirus (LV) expressing miR-135b, without or with MMP-2 siRNAs. 72h later, the expression of MMP-2 was detected by qRT-PCR. (d) Cell invasion assay was performed to investigate the invasion ability of MMP-2 knockdown in miR-135b-overexpressing cells as described in (c). (e) Cell invasion assay was performed to investigate the function of RECK and MMP-2 in invasion repression caused by GAS5. Data represent the mean±SD from three independent experiments. : P<0.05, : P<0.01, and : P<0.001. Scale bar: 50μm.

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