Delivery of miRNA-29b Using R9-LK15, a Novel Cell-Penetrating Peptide, Promotes Osteogenic Differentiation of Bone Mesenchymal Stem Cells

Figure 2

Transfection efficiency of R9-LK15/miR-29b nanocomplexes. (a) Confocal microscopy images of BMSCs treated with FAM-labeled miR-29b (50 nM) alone or complexed with Lipo or R9-LK15. Untreated cells (Con) and those treated with Lipo and R9-LK15 served as controls. Scale bar: 20 μm. (b) Flow cytometric analysis of uptake of FAM-labeled miR-29b by BMSCs. (c) Fluorescence intensity of FAM in confocal microscopy images. Data are the mean ± SD of triplicate experiments. < 0.001.

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