Delivery of miRNA-29b Using R9-LK15, a Novel Cell-Penetrating Peptide, Promotes Osteogenic Differentiation of Bone Mesenchymal Stem Cells
Figure 5
Effects of R9-LK15/miR-29b nanocomplexes on expression of osteogenesis-related genes and proteins. (a) RT-PCR analysis of ALP mRNA expression in untreated BMSCs (Con) and those treated with miR-29b, R9-LK15, Lipo/miR-29b nanocomplexes, and R9-LK15/miR-29b nanocomplexes for 7 days. (b) RT-PCR analysis of HDAC4 mRNA expression in untreated BMSCs (Con) and those treated with miR-29b, R9-LK15, Lipo/miR-29b nanocomplexes, and R9-LK15/miR-29b nanocomplexes for 7 days. (c) Western blot analysis of HDAC4 protein expression in untreated BMSCs (Con) and those treated with miR-29b, R9-LK15, Lipo/miR-29b nanocomplexes, and R9-LK15/miR-29b nanocomplexes. (d) Quantification of relative HDAC4 protein expression using ImageJ. Data are the mean ± SD of triplicate experiments. < 0.05, < 0.01.