PPIA, HPRT1, and YWHAZ Genes Are Suitable for Normalization of mRNA Expression in Long-Term Expanded Human Mesenchymal Stem Cells

Figure 2

Examination of primer specificity, amplicon size and Ct values of selected reference genes. (a) Melting curves and agarose gel images of PCR amplification products. Specificity of primers and amplicon lengths of 10 selected reference genes (RGs) were analyzed by melting curves and agarose gel electrophoresis (bottom of each reference gene melting curve). Lanes in agarose gel images, which were displayed from the left to the right, E-BM-MSCs, M-BM-MSCs, L-BM-MSCs, E-UCB-MSCs, M-UCB-MSCs, L-UCB-MSCs, and nontemplate control. (b) The Ct values of 10 selected RGs in long-term expanded BM-MSCs and UCB-MSCs. The data were presented as mean ± SD. P < 0.05 vs E-BM-MSCs and P < 0.05 vs E-UCB-MSCs.

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