|
| Information of primers | Standard curve parameters |
| Gene name (Symbol) | Sequence | Pro. size | Reference | R2 | M | B | E |
|
| 18S ribosomal RNA (18S) | F: CACGGACAGGATTGACAGATTG
R: AACCAGACAAATCGCTCCAC | 92 | X03205.1 | 0.998 | -3.507 | 34.251 | 0.95 |
|
| Hypoxanthine phosphoribosyltransferase 1 (HPRT1) | F: CGAGATGTGATGAAGGAGATGG
R: TGATGTAATCCAGCAGGTCAGC | 132 | NM_000194.2 | 0.999 | -3.499 | 32.221 | 1.01 |
|
| Beta-2-microglobulin (B2M) | F: CAGCGTACTCCAAAGATTCAGG
R: GGATGAAACCCAGACACATAGC | 116 | NM_003406.3 | 0.995 | -3.452 | 37.651 | 1.01 |
|
| Eukaryotic translation elongation factor 1 alpha 1 (EEF1A1) | F: ACTATCATTGATGCCCCAGGAC
R: ACACCAGCAGCAACAATCAG | 121 | NM_001402.5 | 0.991 | -3.523 | 35.587 | 0.97 |
|
| Ribosomal protein, large, P0 (RPLP0) | F: TGGGCAAGAACACCATGATG
R: CGGATATGAGGCAGCAGTTTC | 98 | NM_001002.3 | 0.998 | -3.511 | 37.016 | 0.96 |
|
| Peptidylprolyl isomerase A (PPIA) | F: TGCTGGACCCAACACAAATG
R: AACACCACATGCTTGCCATC | 89 | NM_021130.3 | 0.990 | -3.388 | 33.650 | 1.03 |
|
| Actin. Beta (ACTB) | F: ACACCAGCAGCAACAATCAG
R: CGGATATGAGGCAGCAGTTTC | 126 | NM_001002.3 | 0.997 | -3.447 | 36.891 | 0.99 |
|
Tyrosine 3-monooxygenase/tryptophan 5-
monooxygenase activation protein, zeta (YWHAZ) | F: CGAAGCTGAAGCAGGAGAAG
R: TTTGTGGGACAGCATGGATG | 111 | NM_003406.3 | 0.995 | -3.356 | 34.613 | 0.94 |
|
| Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | F: ACGAAGCTGAAGCAGGAGAAG
R: GGATGAAACCCAGACACATAGC | 105 | NM_002046.4 | 0.996 | -3.486 | 39.012 | 0.93 |
|
| TATA box binding protein (TBP) | F: AACCAGACAAATCGCTCCAC
R: GGATGGGCATCATGGAAAC | 114 | NM_003406.3 | 0.996 | -3.374 | 33.282 | 1.03 |
|
| Octamer-binding transcription factor 4 (OCT4) | F: ACATCAAAGCTCTGCAGAAAGA
R: AATACCTTCCCAAATAGAACCC | 127 | NM_002701 | - | - | - | - |
|
