The Antiobesity Effects of Buginawa in 3T3-L1 Preadipocytes and in a Mouse Model of High-Fat Diet-Induced Obesity
Figure 1
Bugi inhibits the adipocyte differentiation on 3T3-L1 cells. (a) The cell viability of Bugi on 3T3-L1 adipocytes. (b) The time scheme of Oil Red O staining. (c) The postconfluent 3T3-L1 cells were differentiated in absence or presence of Bugi (62.5, 125, or 250 μg/mL). The lipid accumulation was measured by Oil Red O staining. (d) The lipid content was quantified by measuring absorbance. (e) The protein expression of PPARγ, C/EBPα, SREBP1, and (f) C/EBPβ was measured on Day 2 or Day 4 after the stimulation of 3T3-L1 differentiation, respectively. Densitometric analysis was performed using ImageJ ver. 1.50i. The values represent the mean ± S.D. ###p < 0.001 vs. the control cells; p < 0.05, p < 0.01, and p < 0.001 vs. the differentiated cells.