Protective Effect of Astragaloside IV on Hepatic Injury Induced by Iron Overload

Figure 4

Immunofluorescence was used to measure the expression pattern of LC3 in Lo2 cells. Confocal microscope was used to detect the fluorescence. Green fluorescence intensity was positively correlated with LC3 protein expression and blue fluorescence represented the location of the nucleus of each Lo2 cell. The control group was given 1 DMSO, and the model group was given Fe alone. The treatment group was given different concentrations of Astragaloside IV followed with the 60μM Fe. Scale is 100 μm.